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Title 

Inhibition of starch biosynthesis by antisense expression of cDNAs encoding ADP-glucose pyrophosphorylase small subunit in sweet potato

 

고구마에서 ADP-glucose pyrophosphorylase small subunit cDNA의 Antisense 발현에 의한 전분생합성 저해

Authors 

Sung Ran MinJ M BaeC H HarnWon Joong ChungY B LeeJang Ryol Liu

Publisher 

Korea Society of Plant Biotechnology

Issue Date 

2007

Citation 

Journal of Plant Biotechnology, vol. 34, no. 4, pp. 277-283

Abstract 

Embryogenic calluses derived from shoot apical meristem explants of sweet potato were subjected to particle bombardment to generate transgenic plants for antisense expression of cDNAs encoding two different AGPase small subunit (ibAGP1 and ibAGP2). Plants were generated via somatic embryogenesis. PCR and Southern analysis demonstrated that the incorporation of ibAGP1 and ibAGP2 into the genome in an antisense orientation. Immunoblot analysis confirmed reduced levels of AGPase small subunit in transgenic plant leaves. Plants with both ibAGP1 and ibAGP2 produced a lower level of the protein than plants with ibAGP1 alone. Iodine test demonstrated that transgenic plant leaves and storage root accumulated reduced amounts of starch. Iodine staining of leaf tissues indicated that transgenic plants accumulated less amount of starch than control. In accordance with western blot analysis, plants with both ibAGP1 and ibAGP2 accumulated a lower amount of starch than plants with ibAGP1 alone. Both transgenic plants exhibited a severely retarded growth, resulting in bare survival. It is suggested that disrupted expression of the gene encoding AGPase small subunit is lethal to the growth of sweet potato contrast to other species including potato.

ISSN 

1229-2818

Link 

http://dx.doi.org/10.5010/JPB.2007.34.4.277

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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