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Title 

Induction of axenic culture of Arthrospira (Spirulina) platensis based on antibiotic sensitivity of contaminating bacteria

Authors 

Gang Guk ChoiM S BaeChi-Yong AhnHee-Mock Oh

Publisher 

Springer Verlag (Germany)

Issue Date 

2008

Citation 

Biotechnology Letters, vol. 30, no. 1, pp. 87-92

Keywords 

antibioticsarthrospira platensisaxenic culturecyanobacteriaimipenemneomycinbacteriafilamentous cyanobacteriacell culture

Abstract 

Arthrospira platensis SAG 21.99 and the isolated bacteria (Halomonas spp., Staphylococcus sp., etc.) from the culture of A. platensis SAG 21.99 were treated with five antibiotics to determine the minimal lethal concentrations. The combination of a washing step and a consecutive treatment with antibiotics, imipenem (100 μg ml-1), neomycin (100 μg ml-1) and cycloheximide (20 μg ml-1), treatment step was highly effective in eliminating bacteria. An axenic culture of A. platensis SAG 21.99 could be induced within 3 days using this method. This technique is a simple and rapid method for obtaining axenic cultures of filamentous cyanobacteria.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1007/s10529-007-9523-2

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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