상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of ScpB (Rv1710) from Mycobacterium tuberculosis

Authors 

S Y KwonB S KangMyung Hee KimK J Kim

Publisher 

International Union of Crystallography

Issue Date 

2007

Citation 

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, vol. 63, no. 12, pp. 1058-1060

Keywords 

ScpBsegregation and condensation proteinscrystallizationgene expressionisolation and purificationmolecular cloningmycobacterium tuberculosisX ray crystallographycloning, molecularcrystallography, X-ray

Abstract 

Structural maintenance of chromosome (SMC) proteins play diverse roles in cellular DNA reassembly by directly interacting with DNA. They require non-SMC proteins for their proper function; these include the conserved segregation and condensation proteins (Scps) in prokaryotes. ScpB from Mycobacterium tuberculosis was crystallized using the sitting-drop vapour-diffusion method in the presence of 2 M NaCl and 10% PEG 6000 at 295 K. X-ray diffraction data were collected to a maximum resolution of 2.3 ? at a synchrotron beamline. The crystal belongs to the hexagonal space group R32, with unit-cell parameters a = b = 136.69, c = 78.55 ?, γ = 120°. With one molecule per asymmetric unit, the crystal volume per unit protein weight (V M) is 2.95 ?3 Da-1. The structure was solved by the single anomalous dispersion method and structure refinement is in progress.

Citation 

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 63(12): 1058-1060

ISSN 

1744-3091

Link 

http://dx.doi.org/10.1107/S1744309107056953

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


Files in This Item: SizeFormat
7366.pdf267KbAdobe PDF
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)