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Title 

Genetic discrimination between Catharanthus roseus cultibars by metabolic fingerprinting using 1H NMR spectra of aromatic compounds

Authors 

Suk Weon KimS H BanSoon Chun JeongH J ChungS M KoO J YooJang Ryol Liu

Publisher 

Springer Verlag (Germany)

Issue Date 

2007

Citation 

Biotechnology and Bioprocess Engineering, vol. 12, no. 6, pp. 646-652

Keywords 

amplified fragment length polymorphismmadagascar periwinklepattern recognitionprincipal component analysisproton nuclear magnetic resonance spectroscopy (H NMR)random amplified polymorphic DNA markerscatharanthus roseusphylogenetic tree

Abstract 

When whole cell extracts are subjected to proton nuclear magnetic resonance spectroscopy (1H NMR), metabolite profiles are generated that contain overlapping signals of the majority of compounds within the extract. In order to determine whether pattern recognition based on the metabolite profiles of higher plants is able to genetically discriminate between plants, we analyzed leaf samples of eight cultivars of Catharanthus roseus by 1H NMR. Hierarchical dendrograms, based on the principal component analysis of the 1H NMR total, aliphatic, carbohydrate, and aromatic region data, revealed possible relationships between the cultivars. The dendrogram based on the aromatic region data was in general agreement with the genetic relationships determined by conventional DNA fingerprinting methods. Secologanin and polyphenols were assigned to the signals of the 1H NMR spectra, and contributed most profoundly to the discrimination between cultivars. The overall results indicate that the genetic relationships between C. roseus cultivars are reflected in the differences of the aromatic compounds in the leaves.

ISSN 

1226-8372

Link 

http://dx.doi.org/10.1007/BF02931081

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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