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Title 

Proteomic analysis of pancreas derived from adult cloned pig

Authors 

Jung Il ChaeYoung Keun ChoS K ChoJ H KimY M HanDeog Bon KooKyung Kwang Lee

Publisher 

Elsevier

Issue Date 

2008

Citation 

Biochemical and Biophysical Research Communications, vol. 366, no. 2, pp. 379-387

Keywords 

apoptosispancreaspigproteomic analysisROSsomatic cell cloningproteomics

Abstract 

The potential medical applications of animal cloning include xenotransplantation, but the complex molecular cascades that control porcine organ development are not fully understood. Still, it has become apparent that organs derived from cloned pigs may be suitable for transplantation into humans. In this study, we examined the pancreas of an adult cloned pig developed through somatic cell nuclear transfer (SCNT) using two-dimensional electrophoresis (2-DE) and Western blotting. Proteomic analysis revealed 69 differentially regulated proteins, including such apoptosis-related species as annexins, lamins, and heat shock proteins, which were unanimously upregulated in the SCNT sample. Among the downregulated proteins in SCNT pancreas were peroxiredoxins and catalase. Western blot results indicate that several antioxidant enzymes and the anti-apoptotic protein were downregulated in SCNT pancreas, whereas several caspases were upregulated. Together, these data suggest that the accumulation of reactive oxygen species (ROS) in the pancreas of an adult cloned pig leads to apoptosis.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2007.11.114

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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