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Title 

Variations in protein glycosylation in Hansenula polymorpha depending on cell culture stage

Authors 

S Y KimJung Hoon SohnY R PyunEui Sung Choi

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2007

Citation 

Journal of Microbiology and Biotechnology, vol. 17, no. 12, pp. 1949-1954

Keywords 

CMCaseglucose oxidaseglycosylationhansenula polymorphacarboxymethylcelluloseglycosylated proteinrecombinant proteincell growthfungal cell cultureprotein expression

Abstract 

A simple way to prevent protein hyperglycosylation in Hansenula polymorpha was found. When glucose oxidase from Aspergillus niger and carboxymethyl cellulase from Bacillus subtilis were expressed under the control of an inducible methanol oxidase (MOX) promoter using methanol as a carbon source, hyperglycosylated forms occurred. In contrast, MOX-repressing carbon sources (e.g., glucose, sorbitol, and glycerol) greatly reduced the extent of hyperglycosylation. Carbon source starvation of the cells also reduced the level of glycosylation, which was reversed to hyperglycosylation by the resumption of cell growth. It was concluded that the proteins expressed under actively growing conditions are produced as hyperglycosylated forms, whereas those under slow or nongrowing conditions are as short-glycosylated forms. The prevention of hyperglycosylation in the Hansenula polymorpha expression system constitutes an additional advantage over the traditional Saccharomyces cerevisiae system in recombinant production of glycosylated proteins.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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