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Title 

Complex formation of p65/RelA with nuclear Akt1 for enhanced transcriptional activation of NF-κB

Authors 

OSong KwonK A KimL HeM JungS J JeongJong Seog AhnBo Yeon Kim

Publisher 

Elsevier

Issue Date 

2008

Citation 

Biochemical and Biophysical Research Communications, vol. 365, no. 4, pp. 771-776

Keywords 

AktKi-RasNF-κBproteasomeimmunoglobulin enhancer binding proteinproteasome inhibitorprotein farnesyltransferase inhibitorprotein kinase Btranscription factor RelAmultiprotein complexs

Abstract 

Akt1 was revealed to interact with Ki-Ras in the cytoplasm of Ki-Ras-transformed human prostate epithelial cells, 267B1/K-ras. Moreover, p65/RelA in the nucleus was found to interact with both Ki-Ras and Akt1, suggesting the nuclear translocation of Akt1:Ki-Ras complex for NF- κB activation. In support of this, compared with wild type Akt1, the dominant negative Akt1 mutant was decreased in its nuclear expression, reducing the Ki-Ras-induced NF-κB transcriptional activation. Moreover, inhibitors of Ras (sulindac sulfide and farnesyltransferase inhibitor I) or PI3K/Akt (wortmannin), reduced the amounts of Akt1 and Ki-Ras in the nucleus as well as partial NF-κB activity. The complete inhibition of Ki-Ras-induced NF-κB activation, however, could only be obtained by combined treatment with wortmannin and proteasome inhibitor-1. Accordingly, clonogenic assay showed Akt1 contribution to IκBα-mediated NF-κB activation for oncogenic cell growth by Ki-Ras. Our data suggest a crucial role of Ki-Ras:Akt1 complex in NF-κB transcriptional activation and enhancement of cell survival.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2007.11.037

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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