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Title 

Constitutive coexpression of Bacillus endoxylanase and Trichoderma endoglucanase genes in Saccharomyces cerevisiae

Authors 

J H LeeM Y LimM J KimSun Yeon HeoJ H SeoY H KimS W Nam

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2007

Citation 

Journal of Microbiology and Biotechnology, vol. 17, no. 12, pp. 2076-2080

Keywords 

coexpressionendoglucanaseendoxylanaseS. cerevisiaexylanbacillusgene expressiongene insertiongene sequencegenetic transformation

Abstract 

The endoxylanase (GenBank Access No. U51675) of Bacillus spp. and endoglucanase (GenBank Access No. AY466436) of Trichoderma spp. were separately inserted downstream of the yeast constitutive ADH1 promoter, resulting in three different plasmids (pAGX1, pAGX2, and pAGX3) according to the transcription direction of two genes. When the yeast transformants, S. cerevisiae SEY2102 harboring each expression plasmid, were grown on YPD medium, the total activities of the enzymes were approximately 3.01 unit/ml, 3.24 unit/ml, and 7.56 unit/ml for endoxylanase and 0.60 unit/ml, 0.54 unit/ml, and 0.39 unit/ml for endoglucanase, in the following order: the pAGX1, pAGX2, and pAGX3. More than 70% of the endoxylanase and endoglucanase activities was found in the extracellular media.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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