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Title 

Screening of the genes expressed in Pichia pastoris grown in phosphate-limited chemostat culture

 

인산제한상태에서 발현되는 Pichia pastoris 유래 유전자 탐색

Authors 

Ji Yeon HongJungoh AhnM S ParkS Y ChoiEui Sung ChoiJoon Ki JungHong-Weon Lee

Publisher 

The Korean Society for Applied Microbiology and Biotechnology

Issue Date 

2007

Citation 

Korean Journal of Microbiology & Biotechnology, vol. 35, no. 4, pp. 272-277

Keywords 

phosphate limitationpichia pastorissodium-phosphate (Na+/Pi) symporter (NPS)glyceraldehyde 3 phosphate dehydrogenasemannose phosphate isomerasephosphateproton transporting adenosine triphosphate synthasesodium phosphate cotransportertriosephosphate isomerasechemostat

Abstract 

The physiological responses of microorganisms to specific nutrient limitation can be regulated at the transcriptional levels. In this study, in order to develop the Pichia pastoris-derived promoter inducible by nutrient-limited condition, we constructed cDNA libraries using RT-PCR of total RNA from P. pastoris in steady-states of phosphate-limited chemostat with different dilution rates. Various genes were detected from cDNA library. Among these genes, the gene encoding putative sodium/phosphate (Na+/Pi) symporter (NPS), high affinity transporter of phosphate, was detected. It was observed that expression of NPS increased in a manner specific to phosphate-limited condition through Northern blot. Therefore, it is thought that the promoter from NPS gene may have the potential as auto-inducible promoter by phosphate-limited culture condition without inducer.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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