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Title 

Immunolocalization of anion exchanger 1 (Band 3) in the renal collecting duct of the common marmoset

Authors 

J H SongY H KimT C KangM H WonJ G SuhByung Hwa HyunY S OhS Y RyuJ Y Jung

Publisher 

The Korean Society of Veterinary Science

Issue Date 

2007

Citation 

Journal of Veterinary Science, vol. 8, no. 4, pp. 329-333

Keywords 

band 3 proteinkidneymarmosetmonkeyanion exchange protein 1, erythrocyte

Abstract 

The purpose of this study was to determine the expression and distribution of band 3 in the collecting duct and connecting tubules of the kidney of the marmoset monkey (Callithrix jacchus), and to establish whether band 3 is expressed in type A intercalated cells. The intracellular localization of band 3 in the different populations of intercalated cells was determined by double-labeling immunohistochemistry. Immunohistochemical microscopy demonstrated that band 3 is located in the basolateral plasma membranes of all type A intercalated cells in the connecting tubule (CNT), cortical collecting duct (CCD), and outer medullary collecting duct (OMCD) of the marmoset. However, type B intercalated cells and non-A/non-B intercalated cells did not show band 3 labeling. Electron microscopy of the CNT, CCD and OMCD confirmed the light microscopic observation of the basolateral plasma membrane staining for band 3 in a subpopulation of interacted cells. Basolateral staining was seen on the plasma membrane and small coated vesicles in the perinuclear structure, some of which were located in the Golgi region. In addition, there was no labeling of band 3 in the mitochondria of the CNT, CCD and in OMCD cells. The intensity of the immunostaining of the basolateral membrane was less in the CNT than in the CCD and OMCD. In contrast, band 3 immunoreactivity was greater in the intracellular vesicles of the CNT. From these results, we suggest that the basolateral Cl-/HCO3- exchanger in the monkey kidney is in a more active state in the collecting duct than in the CNT.

URI 

https://doi.org/10.4142/jvs.2007.8.4.329

ISSN 

1229-845X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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