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Title 

Protective effect of oxidative stress in HaCaT keratinocytes expressing E7 oncogene

Authors 

J H ShimK H KimY S ChoH S ChoiEun Young SongP K MyungJ S KangS K SuhS N ParkD Y Yoon

Publisher 

Springer Verlag

Issue Date 

2008

Citation 

Amino Acids, vol. 34, no. 1, pp. 135-141

Keywords 

apoptosis signalE7human papillomavirusoncogeneoxidative stresskeratinocytespapillomavirus E7 proteins

Abstract 

In a previous study, we established a stable cell line which constitutively expresses E7 in HaCaT human keratinocyte cell line and identified various relevant factors including oxygen modulators affected by the E7 oncogene. E7-expressing HaCaT cells (HaCaT/E7) appeared to be more resistant to H 2O2-induced cell death. Here, we demonstrate how E7 oncogene would modulate oxidative stress-induced cell death. In addition, we verified the increased expression of catalase in the HaCaT/E7 by Western blot analysis. The results suggest that the E7 oncogene would induce higher resistance to ROS-induced cell injury in the E7-infected cells via the upregulation of catalase. To investigate these paradoxical effects of high concentrations of H2O2 (500 μM-1 mM), we examined their effects on receptor mediated apoptosis, cell death via the mitochondrial pathway and modulation of apoptosis related factors. Our results revealed that HaCaT keratinocytes infected with HPV 16 E7 oncogene modulated expressions of catalase, Bcl-xL, IL-18, Fas, Bad, and cytochrome c as well as NF-κB, resulting in the resistance to oxidative stress-induced cell death.

ISSN 

0939-4451

Link 

http://dx.doi.org/10.1007/s00726-007-0499-y

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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