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Title 

Assessment of the persistence of DNA in decomposing leaves of CMVP0-CP transgenic chili pepper in the field conditions

 

포장조건에서 CMVP0-CP형질전환 고추 도입유전자의 지속성 조사

Authors 

BumKyu LeeChang-Gi KimJi Young ParkKee Woong ParkHoonbok YiC H HarnHwan Mook Kim

Issue Date 

2007

Citation 

Korean Journal of Environmental Agriculture, vol. 26, no. 4, pp. 319-324

Keywords 

DNA persistencechili peppergene transfersoiltransgenic plant

Abstract 

This study was conducted to evaluate the persistence of DNA in the transgenic chili pepper resistant to cucumber mosaic virus (CMV) in the field condition. We analyzed the persistence of genes in the leaf samples obtained from two field conditions, below and above soil. Transgenic and non-transgenic leaf tissues were buried in the soil at a depth of 10 cm or placed on the soil surface. Qualitative and quantitative PCR analysis showed that the amount of transferred genes from the transgenic peppers below and above soil was dropped to 28.3-42.7% one month after buried and it was rapidly reduced to 0.9-3.3% after two months. The transgenes were not detected three to four month after buried. In addition, DNA of the leaves placed below soil decomposed about 8%more than those on soil surface. The gene transfer from decomposing leaves of the transgenic pepper to soil was investigated by PCR analysis with the soil attached to the samples. The PCR result indicated that the gene transfer from the transgenic pepper to soil was not occurred.

ISSN 

1225-3537

Link 

http://dx.doi.org/10.5338/KJEA.2007.26.4.319

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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