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Title 

Two gap junction channel (innexin) genes of the Bombyx mori and their expression

Authors 

S M HongS W KangT W GooNam-Soon KimJ S LeeK A KimS K Nho

Publisher 

Elsevier

Issue Date 

2008

Citation 

Journal of Insect Physiology, vol. 54, no. 1, pp. 180-191

Keywords 

bombyx morigap junction proteinsinnexin familyinnexinsinsect embryonic developmentgap junction proteingene expressiongeneticsmolecular geneticsbombyx

Abstract 

Gap junctions are clusters of intercellular channels that are associated with embryonic development and neural signaling. Innexins, invertebrate gap junction proteins, have been identified in Drosophila and Caenorhabditis. Here, we report the isolation and characterization of two novel members of the insect innexin family, Bm inx2 and Bm inx4, from embryos of the silkworm, Bombyx mori, during the germ-band formation stage. Bm inx2 is a single copy gene with one exon, while Bm inx4 is a single copy gene with four exons and three introns. The predicted proteins show structural similarities with other innexin family members, including four transmembrane (TM) domains, two extracellular loops (ELs), one cytoplasmic loop (CL), and typical conserved amino acids. Bm inx2 is phylogenetically orthologous to the other insect inx2 genes, but Bm inx4 is not orthologous to any known innexin including Dm inx4. Interestingly, Northern blotting and in situ hybridization showed that Bm inx2 was variously expressed across all developmental stages and in various tissues, with high expression seen in the nervous system at the time of embryogenesis. In contrast, Bm inx4 was transiently expressed at the germ-band formation stage of embryogenesis, and was specifically expressed in the ovary and testis during the larval and pupal stages. The isolation and characterization of these novel genes should form the basis for further study of the functional events that occur during development and neuronal communication in B. mori.

ISSN 

0022-1910

Link 

http://dx.doi.org/10.1016/j.jinsphys.2007.09.002

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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