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Title 

Epigenetic down-regulation and suppressive role of DCBLD2 in gastric cancer cell proliferation and invasion

Authors 

Mi-rang KimK T LeeHay-Ran JangJeong Hwan KimS M NohK S SongJ S ChoH Y JeongSeon-Young KimHyang Sook YooYong Sung Kim

Publisher 

American Association for Cancer Research

Issue Date 

2008

Citation 

Molecular Cancer Research, vol. 6, no. 2, pp. 222-230

Keywords 

protein dcbld2cancer cellcancer cell culturecancer invasioncell proliferationdown regulationepigeneticsgenetic regulationhuman cellstomach cancer

Abstract 

The promoter region of Discoidin, CUB and LCCL domain containing 2 (DCBLD2) was found to be aberrantly methylated in gastric cancer cell lines and in primary gastric cancers, as determined by restriction landmark genomic scanning. DCBLD2 expression was inversely correlated with DCBLD2 methylation in gastric cancer cell lines. Treatment with 5-aza-2′-deoxycytidine and trichostatin A partially reversed DCBLD2 methylation and restored gene expression in DCBLD2-silenced cell lines. In an independent series of 82 paired gastric cancers and adjacent normal tissues, DCBLD2 expression was down-regulated in 79% of gastric cancers as compared with normal tissues as measured by real-time reverse transcription-PCR. Pyrosequencing analysis of the DCBLD2 promoter region revealed abnormal hypermethylation in gastric cancers, and this hypermethylation was significantly correlated with down-regulation of DCBLD2 expression. Furthermore, ectopic expression of DCBLD2 in gastric cancer cell lines inhibited colony formation in both anchorage-dependent and anchorage-independent cultures and also inhibited invasion through the collagen matrix. These data suggest that down-regulation of DCBLD2, often associated with promoter hypermethylation, is a frequent event that may be related to the development of gastric cancer.

ISSN 

1541-7786

Link 

http://dx.doi.org/10.1158/1541-7786.MCR-07-0142

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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