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Title 

Identification of replicative senescence-associated genes in human umbilical vein endothelial cells by an annealing control primer system

Authors 

T W KimH J KimC H LeeH Y KimS H BaekJ H KimKi Sun KwonJ R Kim

Publisher 

Elsevier

Issue Date 

2008

Citation 

Experimental Gerontology, vol. 43, no. 4, pp. 286-295

Keywords 

cell agingDDRT-PCRendothelial cellsgene expressionbone morphogenetic protein 4cell cycle protein 37gene productrecombinant human micaannealing control primer

Abstract 

Cellular senescence is regulated by specific genes in many organisms. The identification and functional analysis of senescence-associated genes could provide valuable insights into the senescence process. Here, we employed a new and improved differential display reverse transcription-polymerase chain reaction (DDRT-PCR) method that involves annealing control primers (ACPs) to identify genes that are differentially expressed in human umbilical endothelial cells during replicative senescence. Using 120 ACPs, we identified 31 differentially expressed genes (DEGs). Basic local alignment search tool (BLAST) search revealed 29 known genes and two unknown genes. Expression levels of the 29 known genes were confirmed by real-time quantitative RT-RCR and by Western blotting for eight of these genes. CD9 antigen, MHC class I chain-related sequence A (MICA) and cell division cycle 37 homolog (CDC37) were up-regulated, and bone morphogenetic protein 4 (BMP4), dickkopf-1 (DKK1), and transcription factor 7-like 1 (TCF7L1) were down-regulated in old cells. Treatment with recombinant human MICA caused a decrease in cell proliferation and an increase in senescence-associated β-galactosidase staining. Further analysis of differentially expressed genes may provide insights into the molecular basis of replicative senescence and vascular diseases associated with cellular senescence.

ISSN 

0531-5565

Link 

http://dx.doi.org/10.1016/j.exger.2007.12.010

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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