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Title 

Thermostable β-glycosidase-CBD fusion protein for biochemical analysis of cotton scouring efficiency

 

CBD융합단백질을 이용한 생화학적 표면분석기술

Authors 

Jae-Seok HaY M LeeSu Lim ChoiJae Jun SongC S ShinJ H KimSeung Goo Lee

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2008

Citation 

Journal of Microbiology and Biotechnology, vol. 18, no. 3, pp. 443-448

Keywords 

β-glycosidasebiochemical analysiscellulose binding domaincotton scouringfusion proteinthermus caldophilusbeta glycosidasebeta glycosidase cellulose binding proteinglycosidasehybrid protein

Abstract 

Multidomain proteins for the biochemical analysis of the scouring efficiency of cotton fabrics were constructed by the fusion of a reporter moiety in the N-terminal and the cellulose binding domain (CBD) in the C-terminal. Based on the specific binding of the CBD of Cellulomonas fimi exoglucanase (Cex) to crystalline cellulose (Avicel), the reporter protein is guided to the cellulose fibers that are increasingly exposed as the scouring process proceeds. Among the tested reporter proteins, a thermostable β-glycosidase (BglA) from Thermus caldophilus was found to be most appropriate, showing a higher applicability and stability than GFP, DsRed2, or a tetrameric β-glucuronidase (GUS) from Escherichia coli, which were precipitated more seriously during the expression and purification steps. When cotton fabrics with different scouring levels were treated with the BglA-CBD and incubated with X-Gal as the chromogenic substrate, an indigo color became visible within 2 h, and the color depth changed according to the conditions and extent of the scouring.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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