상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Dielectrophoretic oocyte selection chip for in vitro fertilization

Authors 

W ChoiJi Su KimD H LeeKyung Kwang LeeDeog Bon KooJ K Park

Publisher 

Springer Verlag (Germany)

Issue Date 

2008

Citation 

Biomedical Microdevices, vol. 10, no. 3, pp. 337-345

Keywords 

dielectrophoresisdielectrophoretic velocityin vitro fertilizationoocyteselectioncell selectionfertilization in vitrolab on a chipoocyteoocytes

Abstract 

This paper reports a new dielectrophoretic separation method of porcine oocytes for in vitro fertilization. Conventional manual selection of oocyte highly depends on the expert's experience and lacks universal standards for identifying the quality of oocyte. In this study, an electrode array chip with castellated shape was developed to evaluate dielectrophoretic velocities of oocytes, under applied bias conditions with an AC 3 V waveform at 1 MHz for 15 s. Based on different dielectrophoresis (DEP) response, the selected group of oocytes that moved showed a better developmental potential than the group of oocytes that stayed, representing a higher rate of blastocyst formation and a lower rate of polyspermic fertilization. In addition, the overall developmental potential of oocytes selected by the DEP device was comparable to that of oocytes selected by conventional manual method. These results demonstrate that the difference in dielectrophoretic velocity can be used to establish an objective criterion for the selection of oocytes. Consequently, this method will open the possibility to develop an automatic tool for oocyte selection, which would be helpful for assisted reproductive technologies such as transgenic and clonal animal production.

ISSN 

1387-2176

Link 

http://dx.doi.org/10.1007/s10544-007-9141-8

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)