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Title 

Targeted gene therapy of LS174 T human colon carcinoma by anti-TAG-72 immunoliposomes

Authors 

K S KimY K LeeJ S KimK H KooHyo Jeong HongY S Park

Publisher 

Nature Publishing Group

Issue Date 

2008

Citation 

Cancer Gene Therapy, vol. 15, no. 5, pp. 331-340

Keywords 

humanized monoclonal antibodyPEG-immunoliposomesTAG-72targeted gene therapyantitumor associated glycoprotein 72endostatinprotein antibodyangiogenesiscolon carcinomacontrolled study

Abstract 

Anti-tumor-associated glycoprotein (TAG)-72 PEG-immunoliposomes (PILs) were prepared by conjugation of Fab′ fragments of recombinant humanized monoclonal antibody, HuCC49, to sterically stabilize unilamellar liposomes (90-110 nm in diameter) to target TAG-72-overexpressing cancer cells. The liposomes consisted of 1-palmitonyl-2-oleoyl-sn-glycerol-3-phosphocholine (POPC), 92 mol percent, O,O′-dymyrisyl-N-lysyl aspartate (DMKD cationic lipid), 4 mol percent, distearoyl-phosphatidyl-ethanolamine-polyethylene glycol 2000 (DSPE-PEG2000), 3 mol percent and DSPE-maleimide (DSPE-PEG 2000-Mal), 1 mol percent. These anti-TAG-72 PILs were able to adhere to the surface of TAG-72-overexpressing LS174 T human colon cancer cells more effectively than conventional liposomes. Also, in vitro gene transfection of the LS174 T cells by the anti-TAG-72 PILs in the presence of a high concentration of fetal bovine serum (up to 60%) was greater than that by conventional cationic lipoplexes. Intravenously administered anti-TAG-72 PILs efficiently localized in the LS174 T tumor tissues, while the non-targeted conventional liposomes did not. Intravenous administration of the anti-TAG-72 PILs containing plasmids encoding antiangiogenic proteins, such as angiostatin K1/3, endostatin and saxatilin, significantly inhibited in vivo growth of LS174 T tumors and angiogenesis in the tumor tissues. These results demonstrated the potential of TAG-72-mediated targeting of immunoliposomes as a modality for systemic gene delivery to human colon cancer cells.

ISSN 

0929-1903

Link 

http://dx.doi.org/10.1038/cgt.2008.11

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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