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Title 

Molecular cloning of peroxidase cDNAs from dehydration-treated fibrous roots of sweetpotato and their differential expression in response to stress

Authors 

Yun Hee KimKyoung Sil YangCha Young KimS H RyuW K SongSuk Yoon KwonHaeng Soon LeeJ W BangSang Soo Kwak

Publisher 

Korean Society for Biochemistry and Molecular Biology

Issue Date 

2008

Citation 

BMB Reports, vol. 41, no. 3, pp. 259-265

Keywords 

dehydrationenvironmental stressfibrous rootperoxidasesweetpotatogene expression profilinggene expression regulationmolecular cloning

Abstract 

Three peroxidase (POD) cDNAs were isolated from dehydration-treated fibrous roots of sweetpotato (Ipomoea batatas) plant via the screening of a cDNA library, and their expressions were assessed to characterize functions of each POD in relation to environmental stress. Three PODs were divided into two groups, designated the basic PODs (swpb4, swpb5) and the anionic PODs (swpa7), on the basis of the pI values of mature proteins. Fluorescence microscope analysis indicated that three PODs are secreted into the extracellular space. RT-PCR analysis revealed that POD genes have diverse expression patterns in a variety of plant tissues. Swpb4 was abundantly expressed in stem tissues, whereas the expression levels of swpb5 and swpa7 transcripts were high in fibrous and thick pigmented roots. Swpb4 and swpa7 showed abundant expression levels in suspension cultured cells. Three POD genes responded differently in the leaf and fibrous roots in response to a variety of stresses including dehydration, temperature stress, stress-associated chemicals, and pathogenic bacteria.

ISSN 

1976-6696

Link 

http://dx.doi.org/10.5483/BMBRep.2008.41.3.259

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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