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Title 

Agrobacterium-mediated genetic transformation of radish (Raphanus sativus L.)

Authors 

M A ChoSung Ran MinS M KoJang Ryol LiuP S Choi

Publisher 

Japanese Society for Plant Cell and Molecular Biology

Issue Date 

2008

Citation 

Plant Biotechnology, vol. 25, no. 2, pp. 205-208

Keywords 

β-glucuronidase (GUS)agrobacteriumhygromycinparomomycintransgenic radish

Abstract 

In order to generate transgenic radish (Raphanus sativus L., cv. Jin Ju Dae Pyong), hypocotyl explants were cultured on Murashige and Skoog medium containing 4mg l-1 AgNO3, 5mg l-1 acetosyringone, 4mg l-1 6-benzyladenine, and 3mg l-1 α-naphthaleneacetic acid in addition to either 10 mg l-1 hygromycin or 100 mg l-1 paromomycin after cocultivation with disarmed Agrobacterium tumefaciens harboring a plant expression binary vector. Explants co-cultivated with A. tumefaciens GV3101 harboring pCAMBIA1301 and A. tumefaciens EHA101 harboring pPTN290 produced putative transgenic adventitious shoots at frequencies of 0.26% and 0.18%, respectively. Northern blot analysis revealed the gus gene transcript was detected in 8 regenerated plants which confirmed their genetic transformation. The transgenic plants were grown to maturity after vernalization in a greenhouse and appeared morphologically normal. Progeny analysis of independent transgenic plants demonstrated that the gus gene was transmitted in a Mendelian pattern in 3 lines, indicating a single copied gene was incorporated into the genome.

ISSN 

1342-4580

Link 

http://doi.org/10.5511/plantbiotechnology.25.205

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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