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Title 

Etoposide-resistant HT-29 human colon carcinoma cells during glucose deprivation are sensitive to piericidin A, a GRP78 down-regulator

Authors 

J H HwangJ Y KimM R ChaIn Ja RyooS J ChooSung Min ChoY TsukumoA TomidaK Shin-YaY I HwangIck Dong YooH R Park

Publisher 

Wiley-Blackwell

Issue Date 

2008

Citation 

Journal of Cellular Physiology, vol. 215, no. 1, pp. 243-250

Keywords 

antineoplastic agentar 054deoxyglucoseetoposideglucosepiericidin aantiinfective agentcaspase 7chaperoneheat shock protein

Abstract 

Glucose deprivation, a pathophysiological cell condition, causes up-regulation of GRP78 and induction of etoposide resistance in human cancer cells. The induction of drug resistance can be partly explained by the fact that GRP78 can block activation of caspase-7 induced by treatment with etoposide. Therefore, downregulating GRP78 expression may be a novel strategy anticancer drug development. Based on that premise, we established a screening program for anticancer agents that exhibit preferential cytotoxic activity for etoposide-resistant cancer cells under glucose-deprived conditions. We recently isolated an active compound, AR-054, from the culture broth of Streptomyces sp., which prevents stress-induced etoposide resistance in vitro. AR-054 was identified as piericidin A, a prototypical compound, by ESI-MS analysis and various NMR spectroscopic methods. Here, we showed that piericidin A suppressed the accumulation of GRP78 protein and was also highly toxic to etoposide-resistant HT-29 cells, with IC50 values for colony formation of 6.4 and 7.7 nM under 2-deoxyglucose supplemented and glucose-deprived conditions, respectively. Interestingly, piericidin A had no effect under normal growth conditions. Therefore, we suggest that piericidin A prevents up-regulation of GRP78, and exhibits cytotoxicity in glucose-deprived HT-29 cells that are resistant to etoposide.

ISSN 

0021-9541

Link 

http://dx.doi.org/10.1002/jcp.21308

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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