상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Transcriptional regulation of GSDML gene by antisense-oriented HERV-H LTR element

 

Antisense바향의 HERV-H LTR로 인한 GSDML유전자의 전사조절 기작 연구

Authors 

Jae Won HuhD S KimD W KangH S HaK AhnY N NohD S MinKyu Tae ChangH S Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2008

Citation 

Archives of Virology, vol. 153, no. 6, pp. 1201-1205

Keywords 

GSDML protein, humangenetic transcriptiongeneticsmolecular geneticspromoter regions (Genetics)trans-activation (Genetics)transcription, genetichominoideahuman endogenous retrovirushuman endogenous retrovirus H

Abstract 

During the course of hominoid evolution, a new transcript variant of the GSDML (gasdermin-like protein) gene was formed by the integration of the antisense-oriented HERV-H (human endogenous retrovirus) LTR (long terminal repeat) element. To investigate regions that are critical for transcriptional regulation of the GSDML gene, we generated seven deletion mutants from a full-length clone (clone 1/630) that includes the HERV-H LTR sequence and compared their expression levels relative to the full-length parental clone using a transient transfection assay. In the transient transfection assay, deletion of the 5′ flanking region (cellular origin) of the HERV-H LTR sequence led to a 4.5-fold increase in expression compared to the full-length clone, while deletion of the U5 region showed a significant decrease in transcriptional activity. Deletion of the 3′ flanking region of the LTR sequence (clone 42/451) showed similar transcriptional activity to a clone missing the 5′ flanking region of cellular origin (clone 42/630). Taken together, these data indicate that the HERV-H LTR sequence (viral origin) positively regulates transcriptional activity of the GSDML gene and that the 5′ flanking region sequence (cellular origin) exerts negative transcriptional regulation.

ISSN 

0304-8608

Link 

http://dx.doi.org/10.1007/s00705-008-0105-y

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)