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Title 

Role of STAT3 as a negative regulator in Mac2-binding protein expression

 

Mac-2 결합단백질 발현에서의 STAT3의 역할

Authors 

Yuk Pheel ParkJong-Tae KimY YangJ S LimD Y YoonJ W KimHee Gu Lee

Issue Date 

2008

Citation 

Korean Journal of Laboratory Medicine, vol. 28, no. 3, pp. 230-238

Keywords 

epidermal growth factormembrane proteinsmall interfering RNASTAT3 proteinSTAT3 protein, humanTAA90K protein, humantelomerasetumor antigendown regulationgenetic transfection

Abstract 

BACKGROUND: Mac-2 binding protein (Mac-2BP) is a secreted glycoprotein from the culture fluid of several human cancer cells, especially breast, lung, and gastric cells. Mac-2BP plays a role in immune response and cell adhesion activity in patients with various cancer and infectious diseases. In this study, we attempted to identify the regulators of Mac-2BP expression at the transcriptional level. METHODS: To determine the effect of epidermal growth factor (EGF) to Mac-2BP expression in gastric cancers, we constructed the different lengths of Mac-2BP promoter plasmids and measured the promoter activity and Mac-2BP expression. In addition to investigating the role of signal transducer and activator of transcription3 (STAT3) or human telomerase reverse transcriptase (hTERT) as a regulator of Mac-2BP, we transfected the small interfering RNA (siRNA) specific for STAT3 or hTERT, and Mac-2BP level was observed by a quantitative ELISA. RESULTS: EGF treatment could suppress the Mac-2BP transcription in HEK293 or gastric cancer cell lines (SNU-638 or AGS). In 5'-deleted promoter experiment, pGL3-Mac Pro-2377 transfected cells showed a decreased luciferase activity compared to pGL3-Mac Pro-2277. We also identified that (-2,366/-2,356) on Mac-2BP promoter is a putative STAT3 binding site and suppression of STAT3 with STAT3 specific siRNA increased the Mac-2BP level, suggesting the role of STAT3 as a negative regulator, in contrast to hTERT, which is known as a positive regulator. CONCLUSIONS: EGF signal is critical for the Mac-2BP expression, and more importantly, STAT3 could work as a negative regulator, while hTERT as a positive regulator in Mac-2BP transcription.

ISSN 

1598-6535

Link 

http://dx.doi.org/10.3343/kjlm.2008.28.3.230

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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