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Title 

Recent advances in immobilization methods of antibodies on solid supports

Authors 

Yongwon JungJin Young JeongBong Hyun Chung

Publisher 

Royal Society of Chemistry

Issue Date 

2008

Citation 

Analyst, vol. 133, no. 6, pp. 697-701

Keywords 

immobilized antibodyrecombinant antibodyantibody combining siteantibody immobilizationantigen antibody reactionimmobilizationantibodies, monoclonalantigen-antibody reactions

Abstract 

Antibody immobilization on a solid support is an essential process for the development of most immune-based assay systems. The choice of the immobilization method greatly affects antibody-antigen interactions on the assay surface. For the past several years, numerous strategies have been reported to control antibody immobilization, mainly by directing the orientation, stability, and density of bound antibodies on different assay platforms. Here we discuss recent developments in antibody immobilization methods with a particular focus on the strengths and limitations of reported approaches, and thereby provide a useful guideline for the selection of suitable antibody coupling procedures.

ISSN 

0003-2654

Link 

http://dx.doi.org/10.1039/b800014j

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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