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Title 

Fluorescent nanoscale detection of biotin-streptavidin interaction using near-field scanning optical microscopy

Authors 

Hyun Kyu ParkA GokarnaJ P HulmeH G ParkBong Hyun Chung

Publisher 

Institute of Physics

Issue Date 

2008

Citation 

Nanotechnology, vol. 19, no. 23, pp. 235103-235110

Keywords 

optical data storageoptical microscopyoptical propertiesscanningbiotin streptavidin interactionnanoscale detectionoptical (PET) (OPET)streptavidin (SA)near field scanning optical microscopybiotin

Abstract 

We describe a nanoscale strategy for detecting biotin-streptavidin binding using near-field scanning optical microscopy (NSOM) that exploits the fluorescence properties of single polydiacetylene (PDA) liposomes. NSOM is more useful to observe nanomaterials having optical properties with the help of topological information. We synthesized amine-terminated 10,12-pentacosadiynoic acid (PCDA) monomer (PCDA-NH2) and used this derivatized monomer to prepare PCDA liposomes. PCDA-NH2 liposomes were immobilized on an aldehyde-functionalized glass surface followed by photopolymerization by using a 254 nm light source. To measure the biotin-streptavidin binding, we conjugated photoactivatable biotin to immobilized PCDA-NH2 liposomes by UV irradiation (365 nm) and subsequently allowed them to interact with streptavidin. We analyzed the fluorescence using a fluorescence scanner and observed single liposomes using NSOM. The average height and NSOM signal observed in a single liposome after binding were ∼31.3 to 8.5 ± 0.5 nm and 0.37 to 0.16 ± 0.6 kHz, respectively. This approach, which has the advantage of not requiring a fluorescent label, could prove highly beneficial for single molecule detection technology.

ISSN 

0957-4484

Link 

http://dx.doi.org/10.1088/0957-4484/19/23/235103

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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