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Title 

Bombyx mori protein disulfide isomerase enhances the production of nuecin, an antibacterial protein

Authors 

T W GooE Y YunS W KimK H ChoiS W KangK KwonKweon YuO Y Kwon

Publisher 

Korean Society for Biochemistry and Molecular Biology

Issue Date 

2008

Citation 

BMB Reports, vol. 41, no. 5, pp. 400-403

Keywords 

bombyx morinuecinprotein disulfide isomerase (PDI)insect proteinnuecin protein, bombyx moriprotein disulfide isomerasebombyxprotein disulfide-isomerase

Abstract 

The insect baculovirus expression vector system (BEVS) is useful for producing biologically active recombinant proteins. However, the overexpressions of foreign proteins using this system often results in misfolded proteins and the formation of protein aggregates. To overcome this limitation, we developed a versatile baculovirus expression and secretion system using Bombyx mori protein disulfide isomerase (bPDI) as a fusion partner. bPDI gene fusion was found to improve the secretions and antibacterial activities of recombinant nuecin proteins. Thus, we conclude that bPDI gene fusion is a useful addition to BEVS for the large-scale production of bioactive recombinant proteins.

ISSN 

1976-6696

Link 

http://dx.doi.org/10.5483/BMBRep.2008.41.5.400

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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