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Title 

dSETDB1 and SU(VAR)3-9 sequentially function during germline-stem cell differentiation in Drosophila melanogaster

Authors 

Jeongheon YoonKyu-Sun LeeJung Sun ParkKweon YuS G PaikYong-Kook Kang

Publisher 

Public Library of Science

Issue Date 

2008

Citation 

Plos One, vol. 3, no. 5, pp. e2234-e2234

Keywords 

drosophila proteinSETDB1 protein, drosophilasuppressor of variegation 3 9 protein, drosophilaanimal cellcell differentiationdrosophila melanogastergerm cellprotein functionstem celldrosophila proteins

Abstract 

Germline-stem cells (GSCs) produce garnetes and are thus true "immortal stem cells". In Drosophila ovaries, GSCs divide asymmetrically to produce daughter GSCs and cystoblasts, and the latter differentiate into germline cysts. Here we show that the histone-lysine methyltransferase dSETDB1, located in pericentric heterochromatin, catalyzes H3-K9 trimethylation in GSCs and their immediate descendants. As germline cysts differentiate into egg chambers, the dSETDB1 function is gradually taken over by another H3-K9-specific methyltransferase, SU(VAR)3-9. Loss of function mutations in dsetdb1 or Su(var)3-9 abolish both H3K9me3 and heterochromatin protein 1 (HP1) signals from the anterior germarium and the developing egg chambers, respectively, and cause localization of H3K9me3 away from DNA-dense regions in most posterior germarium cells. These results indicate that dSETDB1 and SU(VAR)3-9 act together with distinct roles during oogenesis, with dsetcb1 being of particular importance due to its GSC-specific function and more severe mutant phenotype.

ISSN 

1932-6203

Link 

http://dx.doi.org/10.1371/journal.pone.0002234

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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