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Title 

Identification of small molecules inducing apoptosis by cell-based assay using fission yeast deletion mutants

Authors 

Kyung Sook ChungNam-Hui YimS H LeeShin Jung ChoiK S HeoKwang Lae HoeDong Uk KimS GoehleH B KimK B SongHyang Sook YooK H BaeJ SimonMi Sun Won

Publisher 

Springer Verlag (Germany)

Issue Date 

2008

Citation 

Investigational New Drugs, vol. 26, no. 4, pp. 299-307

Keywords 

anti-cancer compoundapoptosisfission yeastdeletion mutantdrug identificationgene deletionhuman celldrug screening assays, antitumor

Abstract 

The cell-based assay using yeast deletion mutants has been recognized as an efficient analysis to discover therapeutic compounds and reveal their mode of action. In this study, S. pombe deletion mutants-based HTS screening was carried out to identify potential anti-cancer agents. The NCI chemical library of 5700 compounds was screened using kit strains, which consisted of S. pombe mutants harboring deletions in genes involved in DNA repair and mitotic control. During the screening, we identified 40 compounds conferring growth inhibition of S. pombe. Their anti-tumorigenic properties were examined by phenotypic effect on S. pombe, flow cytometry and apoptosis analysis of human cancer. Here, we report hit compounds inducing apoptosis for development of anti-cancer agents suggesting that S. pombe deletion mutants are useful in identifying potential anti-cancer agents in human cancer therapeutics.

ISSN 

0167-6997

Link 

http://dx.doi.org/10.1007/s10637-007-9100-5

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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