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Title 

Modulation of hexa-acyl pyrophosphate lipid A population under Escherichia coli phosphate (Pho) regulon activation

 

대장균의 Pho regulon 활성화에 의한 hexa-acyl pyrophosphate lipid A 분자 형성의 조절

Authors 

M G LamarcheSang-Hyun KimS CrepinM MoureN BertrandR E BishopJ D DubreuilJ Harel

Publisher 

American Society for Microbiology

Issue Date 

2008

Citation 

Journal of Bacteriology, vol. 190, no. 15, pp. 5256-5264

Keywords 

bacterial proteinlipid Aphosphatepolypeptide antibiotic agentpyrophosphatevancomycinadaptationbacterial cellbacterial genebacterial strain

Abstract 

Environmental phosphate is an important signal for microorganism gene regulation, and it has recently been shown to trigger some key bacterial virulence mechanisms. In many bacteria, the Pho regulon is the major circuit involved in adaptation to phosphate limitation. The Pho regulon is controlled jointly by the two-component regulatory system PhoR/PhoB and by the phosphate-specific transport (Pst) system, which both belong to the Pho regulon. We showed that a pst mutation results in virulence attenuation in extraintestinal pathogenic Escherichia coli (ExPEC) strains. Our results indicate that the bacterial cell surface of the pst mutants is altered. In this study, we show that pst mutants of ExPEC strains display an increased sensitivity to different cationic antimicrobial peptides and vancomycin. Remarkably, the hexa-acylated 1-pyrophosphate form of lipid A is significantly less abundant in pst mutants. Among differentially expressed genes in the pst mutant, lpxT coding for an enzyme that transfers a phosphoryl group to lipid A, forming the 1-diphosphate species, was found to be downregulated. Our results strongly suggest that the Pho regulon is involved in lipid A modifications, which could contribute to bacterial surface perturbations. Since the Pho regulon and the Pst system are conserved in many bacteria, such a lipid A modification mechanism could be widely distributed among gram-negative bacterial species.

ISSN 

0021-9193

Link 

http://dx.doi.org/10.1128/JB.01536-07

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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