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Title 

Agrobacterium-mediated transformation in Citrullus lanatus

Authors 

M A ChoC Y MoonJang Ryol LiuP S Choi

Publisher 

Springer Verlag (Germany)

Issue Date 

2008

Citation 

Biologia Plantarum, vol. 52, no. 2, pp. 365-369

Keywords 

β-glucuronidaseagrobacterium strainsglufosinateparomomycintransgenic watermeloncitrullus lanatuscitrullus lanatus var. lanatus

Abstract 

Agrobacterium tumefaciens-mediated transformation was used to produce transgenic watermelon. Cotyledonary explants of Citrullus lanatus Thumb (cv. Daesan) were co-cultivated with Agrobacterium strains (LBA4404, GV3101, EHA101) containing pPTN289 carrying with bar gene and pPTN290 carrying with nptII gene, respectively. There was a significant difference in the transformation frequency between bacteria strains and selective markers. The EHA101/pPTN289 showed higher transformation frequency (1.16 %) than GV3101/pPTN289 (0.33 %) and LBA4404/pPTN289 or /pPTN290 (0 %). The shoots obtained (633 and 57 lines) showed some resistance to glufosinate and paromomycin, respectively. of them, the β-glucuronidase positive response and PCR products amplified by bar and nptII specific primers showed at least 21 plants resistant to glufosinate and at least 6 plants to paromomycin. Southern blot analysis revealed that the bar gene integrated into genome of transgenic watermelon. Acclimated transgenic watermelons were successfully transplanted in the greenhouse and showed no phenotypic variation.

ISSN 

0006-3134

Link 

http://dx.doi.org/10.1007/s10535-008-0076-6

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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