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Title 

Lectin precipitation using phytohemagglutinin-L(4) coupled to avidin-agarose for serological biomarker discovery in colorectal cancer

Authors 

Yong Sam KimO L SonJ Y LeeS H KimS OhY S LeeC H KimJ S YooJ H LeeE MiyoshiN TaniguchiS M HanashHyang Sook YooJeong Heon Ko

Publisher 

Wiley-Blackwell

Issue Date 

2008

Citation 

Proteomics, vol. 8, no. 16, pp. 3229-3235

Keywords 

biomarkervolorectal cancerGnT-VL-PHAagaroseavidinlectinphytohemagglutinincolorectal cancerprecipitation

Abstract 

N-acetylglucosaminyltransferase V (GnT-V) has been reported to be upregulated in malignant cancer cells, and its targets have been sought after with regard to biomarker identification. The low capacity and high false positive rates of 2-DE gel-based lectin blots using phytohemagglutinin-L 4 (L-PHA) prompted us to develop a novel protocol for identifying GnT-V targets, in which serum proteins were subjected to immunodepletion, alkylation, and lectin precipitation using L-PHA coupled to avidin-agarose bead complexes, and tryptic digestion. Proteins captured by L-PHA conjugates were analyzed by a nano-LC-FT-ICR/LTQ MS. Here, we report 26 candidate biomarkers for colorectal cancer (CRC) that show 100% specificity and sensitivities of greater than 50%. Not only can these candidate proteins be used as analytes for validation, but the novel protocol described herein can be applied to biomarker discovery in nonCRCs.

ISSN 

1615-9853

Link 

http://dx.doi.org/10.1002/pmic.200800034

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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