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Title 

Disialoganglioside GD3 synthase expression recruits membrane transglutaminase 2 during erythroid differentiation of the human chronic myelogenous leukemia K562 cells

Authors 

S K KangYong Sam KimY J KongK H SongY C ChangY G ParkJeong Heon KoY C LeeC H Kim

Publisher 

Wiley-Blackwell

Issue Date 

2008

Citation 

Proteomics, vol. 8, no. 16, pp. 3317-3328

Keywords 

erythroid differentiationganglioside GD3membrane proteintransglutaminase 2epidermal growth factor receptor 2cell differentiationcell membraneerythroid cellhuman cellleukemia

Abstract 

By employing proteomics analysis tool, we examined the effects of GD3 synthase expression on the differentiation properties of chronic myelogenous leukemia (CML)-derived leukemia cells K562. Forced expression of GD3 synthase induced erythroid differentiation as determined by an increase in glycophorin A expression and synthesis of hemoglobins. The proteomic analysis revealed that 15 proteins were increased by GD3 synthase. In contrast, we observed three protein gel spots decreased in contents in the cell membranes of GD3 synthase-transfected K562 cells. Among the increased proteins, membrane transglutaminase 2 (TG2) was specifically increased in the cell membrane of GD3 synthase-transfected K562 cells. Then, we generated the GD3 synthase-transfected cells in the K562 cells. Interestingly, the TG2 level was increased in GD3 synthase-transfected cells compared with vector- and plasma membrane-associated ganglioside sialidase (Neu3)-transfected cells. In addition, its ability to be photoaffinity-labeled with [α-32P]GTP was also increased in the GD3 synthase- and TG2-transfected cells. Moreover, small interfering RNA (siRNA) analysis for the GD3 synthase showed the decrease or abolishment of the membrane TG2. Finally, GD3 synthase-transfected cells accelerated the erythroid differentiation. Therefore, we propose that the recruitment of TG2 into membranes by GD3 might play an important role in the erythroid differentiation in K562 cells.

ISSN 

1615-9853

Link 

http://dx.doi.org/10.1002/pmic.200800153

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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