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Title 

Nanoscopic observation of a gold nanoparticle-conjugated protein using near-field scanning optical microscopy

Authors 

Hyun Kyu ParkYong Taik LimJin Kyeong KimH G ParkBong Hyun Chung

Publisher 

Elsevier

Issue Date 

2008

Citation 

Ultramicroscopy, vol. 108, no. 10, pp. 1115-1119

Keywords 

gold nanoparticle (AuNP)near-field scanning optical microscopy (NSOM)streptavidin detectionflow interactionsglassoptical microscopyscanningnanoscopic scaleprotein interactionsmicroscopy

Abstract 

This study describes a single gold nanoparticle (AuNP)-based observation of biomolecular interaction using a near-field scanning microscope (NSOM) in transmission mode. To observe streptavidin molecules, a glass surface was first patterned with a micro-scale line of (3-aminopropyl)trimethoxysilane (APTMS) by micro-contact printing (μCP) with a subsequent reaction of N-hydroxysuccinimide (NHS)-biotin. The AuNP-conjugated streptavidin was then applied to the biotin-modified glass surface and NSOM was employed to detect the resulting specific interaction between streptavidin and biotin on the glass surface. Using the optical and topological images generated from the NSOM analysis, the interaction could be observed at the nanoscopic scale. This study demonstrates that the NSOM is a powerful tool for the detection of protein interactions at the nanoscopic level when the protein is conjugated with AuNPs.

ISSN 

0304-3991

Link 

http://dx.doi.org/10.1016/j.ultramic.2008.04.024

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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