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Title 

Mixed self-assembly of polydiacetylenes for highly specific and sensitive strip biosensors

Authors 

Hyun Kyu ParkSang Jeon ChungH G ParkJ H ChoMoonil KimBong Hyun Chung

Publisher 

Elsevier

Issue Date 

2008

Citation 

Biosensors & Bioelectronics, vol. 24, no. 3, pp. 480-484

Keywords 

mixed self-assemblynonspecific bindingpolydiacetylenestrip biosensorbiosensors

Abstract 

Polydiacetylenes (PDAs), which possess unique properties that allow them to change color in response to environmental changes such as variations in pH, temperature, and molecular binding, have been widely investigated as signal transducers in biosensor applications. Most PDA-based sensors reported to date have been evaluated largely on the basis of their ability to detect purified samples, however, and their specificity has rarely been tested. In this study, novel PDAs fabricated on polyvinylidene fluoride (PVDF) strips by photoreaction of composite diacetylene self-assemblies were developed as biosensors, and nonspecific binding to off-target biomolecules was assessed. A mixed PDA surface containing biotin and ethanolamide bound the target, i.e., streptavidin, more specifically than did biotin alone. The optimized PDA biosensor exhibited approximately 2850-fold higher selectivity for streptavidin relative to bovine serum albumin controls. A PDA biosensor that was further prepared showed distinctive signals for the urine of diabetic patients compared to urine samples from healthy/non-diabetic person due to the concentration of microalbuminuria. To our knowledge, this is the first strip-type biosensor fabricated with PDAs and the first PDA-based biosensor that can effectively overcome the problem of nonspecific binding.

ISSN 

0956-5663

Link 

http://dx.doi.org/10.1016/j.bios.2008.06.017

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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