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Title 

Identification of novel retromer complexes in the mouse testis

 

마우스 정소에서 정소특이적인 리트로마 복합체 동정

Authors 

Ekyune KimJae Woong LeeDong Chul BaekSang Rae LeeMyeong Su KimSang-Hyun KimK ImakawaKyu Tae Chang

Publisher 

Elsevier

Issue Date 

2008

Citation 

Biochemical and Biophysical Research Communications, vol. 375, no. 1, pp. 16-21

Keywords 

embryonic developmentendosomeretromer complexspermatogenesisvacuolar protein sortingmousetestismice

Abstract 

A family of vacuolar protein sorting (Vps) proteins, which are components of mammalian retromer complex, has been studied in the mouse. Vps26a is known as a retromer component that plays an important role in embryonic development: however, its cell-type expression and precise role remain to be elucidated. In this study, we identified a new isoform of Vps26a, called Vps26aT, which was expressed specifically in the mouse testis. Diverse expression patterns of Vps26 variants in mouse tissues were determined by Western blot and RT-PCR analyses, and the direct interaction of Vps26aT with Vps35 was also demonstrated by immunoprecipitation and pull-down assay using antibodies raised against each Vps component. Our results revealed that the retromer complex could be formed from different Vps26 isoforms in a tissue-specific manner, resulting in more than two types of the retromer complex, including the Vps26a-Vps29-Vps35, Vps26aT-Vps29-Vps35, and Vps26b-Vps29-Vps35 complexes in mouse tissues.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2008.07.067

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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