상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Lysyl oxidase like 4, a novel target gene of TGF-β1 signaling, can negatively regulate TGF-β1-induced cell motility in PLC/PRF/5 hepatoma cells

Authors 

Dong Joon KimDong-Chul LeeSuk Jin YangJeong Ju LeeEun Mi BaeD M KimS H MinSoo Jung KimD C KangB C SangP K MyungKyung Chan ParkYoung Il Yeom

Publisher 

Elsevier

Issue Date 

2008

Citation 

Biochemical and Biophysical Research Communications, vol. 373, no. 4, pp. 521-527

Keywords 

invasionLOXL4MMP2TGF-β1transforming growth factor beta1cell motilityhepatoma cellgeneextracellular matrixDNA microarray

Abstract 

Transforming growth factor-β1 (TGF-β1) is a multi-functional cytokine involved in the regulation of cell proliferation, differentiation and extracellular matrix formation. In search for novel genes mediating the TGF-β1 function at downstream signaling, we performed a cDNA microarray analysis and identified 60 genes whose expression is regulated by TGF-β1 in the liver cancer cell line PLC/PRF/5. Among them, we report here lysyl oxidase like 4 (LOXL4) as a novel target of TGF-β1 signaling, and provide experimental evidence for its expression regulation and function. LOXL4 was found to be the only member of LOX family whose expression is induced by TGF-β1 in hepatoma cells. Deletion mapping of the LOXL4 promoter indicated that the TGF-β1 regulation of LOXL4 expression is mediated through the binding of AP1 transcription factor to a conserved region of the promoter. This was confirmed by the chromatin immunoprecipitation assay that captured c-Fos-bound chromatin from TGF-β1-treated cells. Forced expression of LOXL4 in PLC/PRF/5 cells resulted in inhibition of cell motility through Matrigel in the presence of TGF-β1 treatment. In parallel, LOXL4 suppressed the expression of laminins and α3 integrin and the activity of MMP2. These results suggest that LOXL4 may function as a negative feedback regulator of TGF-β1 in cell invasion by inhibiting the metabolism of extracellular matrix (ECM) components.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2008.06.071

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)