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Title 

Cloning of rat TARC cDNA and analysis of tissue-specific mRNA expression

Authors 

J I ChaeSeong Kyu JooMyung Kyu LeeJ H ParkJ H ShimKyung Kwang LeeD S Lee

Publisher 

Springer Verlag (Germany)

Issue Date 

2008

Citation 

Molecular Biology +, vol. 42, no. 4, pp. 567-571

Keywords 

CCR4chemokineimmunological regulationmolecular immunoligyTARCrattus

Abstract 

Thymus-and activation-regulated chemokine (TARC) is one that selectively controls the migration of type 2-helper T lymphocytes into inflammatory lesions. TARC is a CC chemokine and plays an essential role in recruiting CC chemokine receptor 4-positive Th2 cells to allergic lesions. We cloned TARC cDNA from rat thymus using RT-PCR. The rat TARC clone contained a full-length open reading frame encoding 93 amino acids that showed 83 and 66% homology with mouse and human homologs, respectively. The expression of TARC mRNA was mainly in the lymphoid organs, for example, the thymus, spleen, and lymph node. The recombinant TARC was expressed in Escherichia coli and purified in an active form. In addition, the purified rat TARC with S-tagged specifically binds to human CCR4 in CD4/CCR4-transfected HOS cells by cell-binding assay using flow cytometry. The TARC cDNA clones obtained in this study will be valuable for future studies on allergic diseases in rats.

ISSN 

0026-8933

Link 

http://dx.doi.org/10.1134/S0026893308040122

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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