상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Transgenic tomatoes expressing human beta-amyloid for use as a vaccine against Alzheimer's disease

Authors 

Jung Won YoumJae Heung JeonH KimY H KimK KoHyouk JoungHyun Soon Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2008

Citation 

Biotechnology Letters, vol. 30, no. 10, pp. 1839-1845

Keywords 

Alzheimer's diseaseedible vaccineimmunogenicitytomato-derived beta amyloidimmune responsesnuclear transformationstransgenic tomatoamyloid beta proteinrecombinant vaccinetransgenic plant

Abstract 

Human β-amyloid (Aβ) is believed to be one of the main components of Alzheimer's disease, so reduction of Aβ is considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for Aβ with tandem repeats. Integration of the human Aβ gene into the tomato genome and its transcription were detected by PCR and Northern blot, respectively. Expression of the Aβ protein was confirmed by western blot and ELISA, and then the transgenic tomato line expressing the highest protein level was selected for vaccination. Mice immunized orally with total soluble extracts from the transgenic tomato plants elicited an immune response after receiving a booster. The results indicate that tomato plants may provide a useful system for the production of human Aβ antigen.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1007/s10529-008-9759-5

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)