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Title 

Fission yeast-based screening to identify putative HDAC inhibitors using a telomeric reporter strain

 

분열효모 시스템의 텔로머릭 리포터 균주를 이용한 HDAC 저해제 스크리닝

Authors 

Kyung Sook ChungJiwon AhnJeong Hae ChoiN H YimC M KangC H KimKyeong LeeH M ParkK B SongMi Sun Won

Publisher 

Springer Verlag (Germany)

Issue Date 

2008

Citation 

Molecules and Cells, vol. 26, no. 1, pp. 93-99

Keywords 

HDAC inhibitorheterochromatinschizosaccharomyces pombetelomereyeast-drug screenantineoplastic agenthistone deacetylasehistone deacetylase inhibitorn decyl 2 (n decylcarbamimidoyl methyldisulfanyl) acetamidineprotein ura4

Abstract 

Transcriptional silencing is regulated by promoter methylation and histone modifications such as methylation and acetylation. We constructed a Schizosaccaromyces pombe reporter strain, KCT120a, to identify modifiers of transcriptional silencing, by inserting the ura4+ gene into a heterochromatic telomere region. Two compounds inhibited the activity of histone deacetylases, induced acetylation of histone H3 and caused apoptotic cell death in HeLa cells. Expression of gelsolin and p21waf1/cip1 also increased, as it does in response to HDAC inhibitors such as TSA. Therefore, these compounds appear to be potent inhibitors of HDACs, and hence potential anti-cancer drugs. Our observations suggest that a yeast cell-based assay system for transcriptional silencing may be useful for identifying histone deacetylase inhibitors and other agents affecting chromatin remodeling.

ISSN 

1016-8478

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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