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Title 

The efficiency of RNA interference in Bursaphelenchus xylophilus

Authors 

J E ParkK Y LeeS J LeeW S OhP Y JeongTaeha WooChang-Bae KimY K PaikH S Koo

Publisher 

Springer Verlag (Germany)

Issue Date 

2008

Citation 

Molecules and Cells, vol. 26, no. 1, pp. 81-86

Keywords 

cytochrome Cheat shock protein 70myosin heavy chainpinewood nematodeRNAitropomyosinbursaphelenchus xylophilusRNA interferenceRNA, helminthRNA, double-stranded

Abstract 

RNA Interference (RNAI) was performed on several essential genes in the pinewood nematode Bursaphelenchus xylophilus, which causes pine wilt disease. Double-stranded RNA (deRNA) was delivered to larvae or adult worms by soaking, electroporation, or microinjection. Soaking and electroporation of L2-L3 stage worms in solutions containing dsRNA for essential genes Induced over 250% lethality after 5 days, and gene-specific phenotypes were observed. This lethality agreed with significant reductions of the targeted transcripts, as assayed by reverse-transcription coupled with real time PCR. Microinjection was the most efficient routs as measured by the hatching rate of F1 embryos, which was reduced by 46%. When adult worms were soaked In dsRNA, lethality was induced in the F1 larvae, revealing the persistence of knockdown phenotypes. The penstrance of the RNA/phenotypes for essential genes was relatively low but consistent indicating that RNA/ should be useful for studying the in vivo functions of B. xylophilus gene products.

ISSN 

1016-8478

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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