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Title 

Enhancement of heterologous production of eicosapentaenoic acid in Escherichia coli by substitution of promoter sequences within the biosynthesis gene cluster

 

생합성 유전자의 프로모터 개량에 의한 이종숙주에서 EPA의 생산량 증대

Authors 

Soo-Jin LeeChul Ho KimPil-Soo SeoOh Suk KwonB K HurJeong Woo Seo

Publisher 

Springer Verlag (Germany)

Issue Date 

2008

Citation 

Biotechnology Letters, vol. 30, no. 12, pp. 2139-2142

Keywords 

biosynthesis gene clustereicosapentaenoic acidescherichia coliheterologous productionLacZ promotershewanella oneidensisbiosynthesisfatty acidsgene expression

Abstract 

To enhance the heterologous production of eicosapentaenoic acid (EPA) in Escherichia coli, the EPA biosynthesis gene cluster from Shewanella oneidensis MR-1 was cloned under the lacZ promoter on a high-copy number plasmid, pBluescript SK(+). The production of EPA was remarkably enhanced yielding levels of up to 7.5% of the total fatty acid content in the recombinant E. coli strain by induction with IPTG, whereas the stimulation of EPA production was abolished by adding glucose into the culture medium, probably due to glucose repression acting on the promoter activity.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1007/s10529-008-9809-z

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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