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Title 

Noninvasive imaging of dendritic cell migration into lymph nodes using near-infrared fluorescent semiconductor nanocrystals

 

근적외선 형광 반도성 나노입자를 이용한 수지상세포의 림프절 이동과정의 비칩습적 이미징

Authors 

Young Woock NohYong Taik LimBong Hyun Chung

Publisher 

Federation of American Society of Experimental Biology (FASEB)

Issue Date 

2008

Citation 

FASEB Journal, vol. 22, no. 11, pp. 3908-3918

Keywords 

fluorescent probesimmunotherapeutic cellsin vivo imagingquantum dotsdendritic celllymph nodeanimal cellcell labelingcell migrationfluorescence activated cell sorting

Abstract 

Effective tracking of immunotherapeutic cells is essential for monitoring the migration of injected cells to the target tissue. Here we report the use of near-infrared (NIR) -emitting fluorescent semiconductor nanocrystals, called quantum dots (QDs), for noninvasive in vivo tracking of dendritic cell (DC) migration into lymph nodes. The effect of QDs on DC viability and maturation was systematically investigated using MTT assays and FACS analysis. We found that the labeling of DCs with QDs had no effect on DC phenotype or maturation potential. Cytokine and migration assays revealed that there were no significant changes in either cytokine production or chemokine-dependent migration of QD-labeled DCs relative to unlabeled cells; in both labeled and unlabeled cells, cytokine production and migratory capacity was increased by stimulation with lipopolysaccharide. Furthermore, QDs did not influence allogenic naive T cell activation or uptake of exogenous antigens. Notably, we also demonstrated that it was possible to track QD-labeled DCs injected into the footpad into popliteal and inguinal lymph nodes using NIR fluorescence. Taken together, our protocols establish the potential of noninvasive in vivo imaging of NIR-emitting QDs for tracking immunotherapeutic cells.

ISSN 

0892-6638

Link 

http://dx.doi.org/10.1096/fj.08-112896

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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