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Title 

Fabrication of CdSe/ZnS quantum-dot-conjugated protein microarrays and nanoarrays

 

양자점입자가 부착된 마이크로/나노어레이 제조

Authors 

A GokarnaS K LeeJ S HwangY H ChoYong Taik LimBong Hyun ChungM Lee

Publisher 

Korean Physical Society

Issue Date 

2008

Citation 

Journal of the Korean Physical Society, vol. 53, no. 5, pp. 3047-3050

Keywords 

dip-pen nanolithographynanoarraysprotein microarraysquantum dots

Abstract 

We demonstrate the fabrication and the detection of protein microarrays and nanoarrays, which are tagged by using colloidal CdSe/ZnS quantum dots (QDs), for the purpose of optical readout of those biochips. A microarrayer and a dip-pen nanolithography technique were used for the fabrication of QD-labeled protein microarrays and nanoarrays, respectively. The optical readout of those biochips was conducted by using a microchip scanner and a confocal scanning microscope in the case of the microarrays while an atomic force microscope was employed for characterizing the nanoarrays. We also report a possible way of reducing the 'doughnut phenomenon' in microarrays by varying the spot size from 300 μm to 100 μm, wherein the fluorescence intensity distribution becomes more uniform with decreasing spot diameter.

ISSN 

0374-4884

Link 

http://dx.doi.org/10.3938/jkps.53.3047

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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