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Title 

High sensitivity detection of 16s rRNA using peptide nucleic acid probes and a surface plasmon resonance biosensor

Authors 

Hyou-Arm JoungNae-Rym LeeSeok Ki LeeJun Hyoung AhnYong Beom ShinH S ChoiC S LeeS KimMin-Gon Kim

Publisher 

Elsevier

Issue Date 

2008

Citation 

Analytica Chimica Acta, vol. 630, no. 2, pp. 168-173

Keywords 

16s rRNAcationic nanoparticlepeptide nucleic acidsurface plasmon resonanceaminesamplificationbiosensorsescherichia colinanoparticlesnanostructures

Abstract 

A signal enhancing method allowing highly sensitive detection of E. coli 16s rRNA was developed using peptide nucleic acid (PNA) as a capture probe and a surface plasmon resonance (SPR) sensor as a detector. 16s rRNA has been used as a genetic marker for identification of organisms, and can be analyzed directly without PCR amplification due to the relatively high number of copies. PNA has a neutral backbone structure, therefore hybridization with 16s rRNA results in the ionic condition being changed from neutral to negative. A cationic Au nanoparticle was synthesized and used for signal amplification by ionic interaction with 16s rRNA hybridized on the PNA probe-immobilized SPR sensor chip. This method resulted in a detection limit of E. coli rRNA of 58.2 ± 1.37 pg mL-1. Using this analytical method, Staphylococcus aureus was detected without purification of rRNA.

ISSN 

0003-2670

Link 

http://dx.doi.org/10.1016/j.aca.2008.10.001

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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