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Title 

Proteolytic activity from an alkali-thermotolerant Streptomyces gulbargensis sp. nov.

Authors 

S G DastagerA DayanandW J LiChang-Jin KimJae Chan LeeDong Jin ParkX P TianQ S Raziuddin

Publisher 

Springer Verlag (Germany)

Issue Date 

2008

Citation 

Current Microbiology, vol. 57, no. 6, pp. 638-642

Keywords 

alkalinityenzyme activitystreptomycesstreptomyces gulbargensissubstrate specificity

Abstract 

Multiple proteases were produced and partially purified from an alkali-thermotolerant novel species of Streptomyces (i.e., Streptomyces gulbargensis DAS 131) after 48 h of growth at 45°C. The enzyme preparation exhibited activity over a broad range of pH (4-12) and temperature (27-55°C). Optimum activity was observed at a pH of 9.0 and a temperature of 45°C. Starch and protease peptone was found to be a good source of carbon and nitrogen to enhance the enzyme activity. Two active zones in the range of 19 to 35 kDa were detected on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).

ISSN 

0343-8651

Link 

http://dx.doi.org/10.1007/s00284-008-9257-y

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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