상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Exploring the effects of carbon sources on the metabolic capacity for shikimic acid production in Escherichia coli using In silico metabolic predictions

 

인실리코대사예측을 통한 다양한 탄소원에 대한 대장균에서 쉬큄산 생산을 위한 대사능력 탐색

Authors 

Jungoh AhnHong-Weon LeeR SahaM S ParkJoon Ki JungD Y Lee

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2008

Citation 

Journal of Microbiology and Biotechnology, vol. 18, no. 11, pp. 1773-1784

Keywords 

carbon sourcesconstraints-based flux analysisescherichia coligenome-scale in silico modelshikimic acid productioncarbonshikimate kinaseshikimic acidgrowth, development and aging

Abstract 

Effects of various industrially important carbon sources (glucose, sucrose, xylose, gluconate, and glycerol) on shikimic acid (SA) biosynthesis in Escherichia coli were investigated to gain new insight into the metabolic capability for overproducing SA. At the outset, constraints-based flux analysis using the genome-scale in silico model of E. coli was conducted to quantify the theoretical maximum SA yield. The corresponding flux distributions fueled by different carbon sources under investigation were compared with respect to theoretical yield and energy utilization, thereby identifying the indispensable pathways for achieving optimal SA production on each carbon source. Subsequently, a shikimate-kinase-deficient E. coli mutant was developed by blocking the aromatic amino acid pathway, and the production of SA on various carbon sources was experimentally examined during 51 batch culture. As a result, the highest production rate, 1.92 mmol SA/h, was obtained when glucose was utilized as a carbon source, whereas the efficient SA production from glycerol was obtained with the highest yield, 0.21 mol SA formed per mol carbon atom of carbon source consumed. The current strain can be further improved to satisfy the theoretically achievable SA production that was predicted by in silico analysis.

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.0700.705

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)