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Title 

High frequency plant regeneration from zygotic-embryo-derived embryogenic cell suspension cultures of watershield (Brasenia schreberi)

Authors 

Oh Myung JinH R NaH K ChoiJang Ryol LiuSuk Weon Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2008

Citation 

Plant Biotechnology Reports, vol. 2, no. 1, pp. 87-92

Keywords 

brasenia schreberiplant regenerationsomatic embryogenisiszygotic embryo culture

Abstract 

An improved protocol for high frequency plant regeneration via somatic embryogenesis from zygotic embryo-derived cell suspension cultures of watershield (Brasenia schreberi) was developed. Zygotic embryos formed pale-yellow globular structures and white friable callus at a frequency of 80% when cultured on halfstrength MS medium supplemented with 0.3 mg l-1 2,4-D. However, the frequency of formation of pale-yellow globular structures and white friable callus decreased slightly with increasing concentrations of 2,4-D up to 3 mg l-1, where the frequency reached *50% of the control. Cell suspension cultures from zygotic embryoderived white friable callus were established using halfstrength MS medium supplemented with 0.3 mg l-1 2,4-D. Upon plating of cell aggregates on half-strength MS basal medium, approximately 8.3% gave rise to somatic embryos and developed into plantlets. However, the frequency of plantlet development from cell aggregates was sharply increased (by up to 55%) when activated charcoal and zeatin were applied. Regenerated plantlets were successfully transplanted to potting soil and grown to normal plants in a growth chamber. The distinctive feature of this study is the establishment of a high frequency plant regeneration system via somatic embryogenesis from zygotic embryo-derived cell suspension cultures of watershield, which has not been previously reported. The protocol for plant regeneration of watershield through somatic embryogenesis could be useful for the mass propagation and transformation of selected elite lines.

ISSN 

1863-5466

Link 

http://dx.doi.org/10.1007/s11816-008-0047-6

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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