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Title 

Analysis and characterization of hepatitis B vaccine particles synthesized from Hansenula polymorpha

Authors 

H S SeoJ S ParkK Y HanK D BaeS J AhnHyun Ah KangJ Lee

Publisher 

Elsevier

Issue Date 

2008

Citation 

Vaccine, vol. 26, no. 33, pp. 4138-4144

Keywords 

covalent multimerglycosylationhepatitis B surface antigenstabilityyeastDNA vaccinehansenulahansenula polymorphahepatitis B vaccinesmacromolecular substances

Abstract 

The biochemical and physical properties of hepatitis B virus (HBV) small surface antigen (S-HBVsAg) from Berna Biotech Korea Corp. were systematically analyzed and characterized. Through various electrophoresis and immunoblotting assay of S-HBVsAg and its proteolytic products, it was confirmed that the S-HBVsAg vaccine particles are present in the form of covalent multimers that are assembled via strong intermolecular disulfide bonds. The S-HBVsAg particles contain no N-glycosylation moiety but some O-glycosidically linked mannoses. Evidently from N-terminus sequencing of both monomers and dimers that are formed by complete and partial reduction, respectively, of the S-HBVsAg particles under reducing SDS-PAGE condition, it is evident that each polypeptide within S-HBVsAg particles has authentic sequence of N-terminus. Denaturation plot shows that the S-HBVsAg vaccine particles were extremely stable especially in the solution with high acidity. This stability property of S-HBVsAg vaccine particles could provide very useful information for the optimization of the downstream process of recombinant S-HBVsAg particles synthesized from yeast cultures.

ISSN 

0264-410X

Link 

http://dx.doi.org/10.1016/j.vaccine.2008.05.070

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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