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Title 

An ISFET biosensor for the monitoring of maltose-induced conformational changes in MBP

Authors 

Hae Jeong ParkSang Gyu KimKyoungsook ParkH K LyuChang-Soo LeeSang Jeon ChungW S YunMoonil KimBong Hyun Chung

Publisher 

Elsevier

Issue Date 

2009

Citation 

FEBS Letters, vol. 583, no. 1, pp. 157-162

Keywords 

biosensorvonformational changeISFETMBPsurface chargemaltosemaltose binding proteinbiological monitoringconformational transition

Abstract 

Here we describe an ion sensitive field effect transistor (ISFET) biosensor, which was designed to monitor directly the surface charge of structurally altered maltose binding protein (MBP) upon stimulation with maltose. This study is the first report of the application of a FET biosensor to the monitoring of conformationally changed proteins. Consequently, a significant drop in current on the basis of the charge-dependent capacitance measurement has been clearly observed in response to maltose, but not for the glucose control, thereby indicating that the substrate-specific conformational properties of the target protein could be successfully monitored using the ISFET. Collectively, our results clearly suggest that ISFET provide a high fidelity system for the detection of maltose-induced structural alterations in MBP.

ISSN 

0014-5793

Link 

http://dx.doi.org/10.1016/j.febslet.2008.11.039

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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