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Title 

NDRG2 suppresses cell proliferation through down-regulation of AP-1 activity in human colon carcinoma cells

Authors 

Young-Jun KimS Y YoonJong-Tae KimS C ChoiJ S LimJ H KimEun Young SongHee Gu LeeIn Pyo ChoiJae Wha Kim

Publisher 

Wiley-Blackwell

Issue Date 

2009

Citation 

International Journal of Cancer, vol. 124, no. 1, pp. 7-15

Keywords 

c-Jun phosphorylationcyclin D1/p21growth retardationcyclin D1cyclin dependent kinase inhibitor 1ANDRG2 protein, humantranscription factor AP 1carcinomacell cyclecell proliferation

Abstract 

Recently, the anti-tumor activity of N-myc downstream-regulated gene 2 (NDRG2) was elucidated, but the molecular mechanism of how NDRG2 works as a tumor suppressor is not well known. To determine the function of NDRG2 as a tumor suppressor, we established stable cell lines expressing NDRG2 protein or its mutant forms, and studied their effects on tumor cell growth. Interestingly, constitutive expression of wild-type NDRG2 induced the growth retardation of SW620 colon carcinoma cells. Introduction of NDRG2 into SW620 cells induced the decrease of c-Jun phos-phorylation at Ser63, followed by the attenuation of activator pro-tein-1 (AP-1) function as a transcriptional activator. Subsequently, the down-regulation of cyclin D1, which is known as a major target for AP-1 transcription activator, resulted in cell cycle arrest at G1/S phase. Additionally, treatment of NDRG2-siRNA on NDRG2-expressing cells has induced the recovery of c-Jun phos- phorylation and cyclin D1 expression. Cell proliferation of those cells was also increased compared with untreated cells. NDRG2 mutants of which the phosphorylation sites at C-terminal region were removed by deletion or site-directed mutagenesis have shown no effect on cyclin D1 expression and could not induce cell growth retardation. In conclusion, NDRG2 modulates intracellular signals to control cell cycle through the regulation of cyclin D1 expression via phosphorylation pathway.

ISSN 

0020-7136

Link 

http://dx.doi.org/10.1002/ijc.23945

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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